Cancer biology – signaling, metabolism and gene expression (RNAseq/microarray) of lung and brain cancer
Cancer Stem cells vs normal stem cells
MicroRNA profiling of brain tumors
Drug/microRNAs delivery using liposomes and nanoparticles
Exploring Himalayan biodiversity for treatment of cancer
Selective targeting of cancer stem cells, the root cause of cancer dissemination, metastasis and/or relapse.
Ph.D. Molecular Medicine (October 2012) Final Viva-Voce-22nd February 2013, Sapienza University of Rome, Department of Molecular Medicine, Rome, Italy. Thesis Advisor: Late Prof. Alberto Gulino, Prof. Elisabetta Ferretti
M.Sc. Biochemistry, July 2008 (2006-2008) Department of Biochemistry, University of Delhi, South Campus, New Delhi, India.
B.Sc. Biochemistry, July 2006 (2003-2006) Department of Biochemistry, Daulat Ram College, University of Delhi, North Campus, New Delhi, India.
Academic Research Experience
Assistant Professor (DST-INSPIRE), IIT Mandi, Himachal Pradesh, India (Nov 2015-Present)
Postdoctoral Research Fellow, McMaster University, Canada (Nov 2013 – October 2015)
Postdoctoral Research Fellow, Sapienza University of Rome, Italy (Nov 2012 – October 2013)
Two Travel Awards (OIRM: Ontario Institute for Regenerative Medicine and SCN: Stem Cell Network) for 2015 Till & McCulloch Meetings, Toronto, Canada and poster presentation in the same
Travel Award for 2014 Till & McCulloch Meetings, Ottawa, Canada and poster presentation in the same
Postdoctoral Fellowship, McMaster University, Hamilton, Canada: November 2013-October 2015
Sapienza Post Doctoral Fellowship, Department of Molecular Medicine, University of Rome 'La Sapienza', Italy: August 2013-October 2013
Marie Curie Fellow, European Union: Aug 2010-July 2013
Qualified GATE 2008 (Graduate aptitude test in engineering) conducted by IIT, Kanpur with 99.89 percentile marks in the subject Life Sciences. Secured 13 All India Rank.
Qualified Joint CSIR-UGC JRF in 2007 (both June and December).
Received interview call letters/shortlisted for prestigious Dr. Shyama Prasad Mukherjee fellowship (SPMF) (two letters from CSIR-UGC and one from GATE).
“Monsanto Scholarship”, during M.Sc., Government of India: 2006-2008
“Silver Jubilee Merit Scholarship” during B.Sc., Government of India: 2003-2006
Delhi University (North Campus) topper in B.Sc. Biochemistry: 2003-2006
Participated and awarded in 4th National Science Olympiad, 2002 (At School Level).
Merit certificates for securing positions at school level in Academics, Olympiads, Quiz and Sports competitions throughout academic career.
Journal Publications (published)
Mohini Singh, Neha Garg, Chitra Venugopal, Robin Hallett, Tomas Tokar, Nicole McFarlane, Sujeivan Mahendram, David Bakhshinyan, Branavan Manoranjan, Parvez Vora, Maleeha Qazi, Carolynn C. Arpin, Brent Page, Sina Haftchenary, David A. Rosa, Ping-Shan Lai, Rodolfo Gómez-Biagi, Ahmed M. Ali, Andrew Lewis, Mulu Geletu, Naresh K. Murty, John A. Hassell, Igor Jurisica, Patrick T. Gunning, Sheila K Singh. STAT3 pathway regulates lung-derived brain metastasis initiating cell capacity through miR-21 activation. Oncotarget. 2015 Jul 25. [Epub ahead of print]
Chitra Venugopal, Robin Hallett, Parvez Vora, Branavan Manoranjan, Sujeivan Mahendram, Maleeha A Qazi, Nicole McFarlane, Minomi Subapanditha, Sara M. Nolte, Mohini Singh, David Bakhshinyan, Neha Garg, Thusyanth Vijayakumar, Boleslaw Lach, John P. Provias, Kesava Reddy, Naresh K Murty, Bradley W. Doble, Mickie Bhatia, John A. Hassell, Sheila K. Singh. Pyrvinium targets CD133 in human glioblastoma brain tumor-initiating cells. Clinical Cancer Research.2015. (Published OnlineFirst July 7, 2015; doi: 10.1158/1078-0432.CCR-14-3147).
Eleni Anastasiadou, Neha Garg, Rachele Bigi, Shivangi Yadav, Antonio Francesco Campese, Caterina Lapenta, Massimo Spada, Laura Cuomo4 Annalisa Botta, Filippo Belardelli, Luigi Frati, Elisabetta Ferretti, Alberto Faggioni, and Pankaj Trivedi. Epstein-Barr virus infection induces miR-21 in terminally differentiated malignant B cells. Int J Cancer. 2015 Feb 20. doi: 10.1002/ijc.29489.
Garg, N#, Vijaykumar, T., Bakhshinyan, D., Venugopal, C., Singh, SK. microRNA regulation of Brain Tumor Initiating Cells in Central Nervous System Tumors. Stem Cells International, 2015 (Stem Cells Int. 2015; 2015: 141793. Published online 2015 May 3. doi: 10.1155/2015/141793 #corresponding author).
Miele E, Buttarelli FR, Arcella A, Begalli F, Garg N, Silvano M, Po A, Baldi C, Carissimo G, Antonelli M, Spinelli GP, Capalbo C, Donofrio V, Morra I, Nozza P, Gulino A, Giangaspero F, Ferretti E. (2014) High-throughput microRNA profiling of pediatric high-grade gliomas. Neuro-Oncology 2014; Jan;16(2):228-40.
Garg N, Agnese Po, Evelina Miele, Antonio Francesco Campese, Federica Begalli, Marianna Silvano, Paola Infante, Carlo Capalbo, Enrico De Smaele, Gianluca Canettieri, Lucia Di Marcotullio, Isabella Screpanti, Elisabetta Ferretti, Alberto Gulino (2013). microRNA-17-92 cluster is a direct Nanog target and controls neural stem cell through Trp53inp1. EMBO J. Oct 30, 2013; 32(21): 2819–2832.
Rosato P*, Anastasiadou E*, Garg N, Lenze D, Boccellato F, Vincenti S, Severa M, Coccia EM, Bigi R, Cirone M, Ferretti E, Campese AF, Hummel M, Frati L, Presutti C, Faggioni A, Trivedi P.(2012) Differential regulation of miR-21 and miR-146a by Epstein-Barr virus-encoded EBNA2. Leukemia 2012 Nov;26(11):2343-52. (*Co-first authors)
Hasan GM*, Garg N*, Dogra E, Surolia R, Ghosh PC. (2011) Inhibition of the Growth of Plasmodium falciparum in Culture by Stearylamine-Phosphatidylcholine Liposomes. J Parasitol Res. 2011; 2011:120462. (*Co-first authors)
Manoranjan B, Garg N, Bakhshinyan D, Singh SK. The Role of Stem Cells in Pediatric Central Nervous System Malignancies. Adv Exp Med Biol. 2015;853:49-68. doi: 10.1007/978-3-319-16537-0_4 (Book Chapter in “Stem Cell Biology in Neoplasms of the Central Nervous System”, Springer Publisher)
David Bakhshinyan, Maleeha Qazi, Neha Garg, Chitra Venugopal, Nicole McFarlane and Sheila K Singh. Chapter: Isolation and Identification of Neural Cancer Stem/Progenitor Cells.
Published Online: 6 MAR 2015, DOI: 10.1002/9781118670613.ch8
Sheila K. Singh, Branavan Manoranjan, Chitra Venugopal, Parvez Vora, Nicole McFarlane, Neha Garg, Mohini Singh, Aneetinder Mann, David Bakhshinyan, Sujeivan Mahendran, and Sandra Dunn. Sox2 identifies the treatment-refractory stem cell population in group 2 medulloblastoma. Neuro-Oncology 16:iii1–iii22, 2014. doi:10.1093/neuonc/nou206.16
David Bakhshinyan, Thusyanth Vijayakumar, Neha Garg, Branavan Manoranjan, Nicole McFarlane, Chitra Venugopal and Sheila Singh. Discovering the treatment refractory BTIC population in group 3 medulloblastoma. Neuro-Oncology 17:iii1–iii40, 2015. doi:10.1093/neuonc/nov061.90
Courses/ Meetings/Presentations (As a Speaker and Poster)
The International Satellite Meeting to the 13th Human Genome Meeting 2008, on “Genomics, Model Organisms and Diseases” organized at National Centre for Biological Sciences (NCBS), Bangalore, India (01-02 October, 2008).
Poster Presentation entitled “Stearylamine Liposomes in the treatment of Malaria” by Neha Garg*, Gulam Mustafa Hasan and Prahlad C Ghosh at 49th Annual Conference of AMI, International Symposium on Microbial Biotechnology: Diversity, Genomics and Metagenomics at New Delhi, India (18th – 20th December 2008).
HEALING Scientific Meeting and Courses on Ethics and Business Development at Rome, Italy (October 20-23, 2010).
Professional Development short course on Stem Cells: A Pathway through the Maze, Department for Continuing Education at University of Oxford, UK (9-10 December 2010).
1st HEALING Summer School on Hedgehog Gli signalling and International Meeting on Hedgehog-Gli Signalling in Regeneration, Stem Cells and Cancer at Kolymbari, Crete, Greece (20-25 June 2011).
The Cambridge Neural Stem Cell Symposium at Cambridge, UK (4 Sept-6 Sept, 2011).
Continuation of Scientific Research Training under EU FP7-ITN in Prof. Fiona Watt Laboratory (Epidermal Skin Cell Biology), to learn the techniques for studying keratinocytes at CRI and CS-CR, Cambridge, UK (7-9 September, 2011).
Poster presentation entitled “Evolutionary conservation of role of microRNAs in neural stem cells” by Neha Garg*, Elisabetta Ferretti and Alberto Gulino at EMBO workshop on “Evolution in the time of genomics” at Venice, Italy (May 7-9, 2012).
Healing scientific meeting and Courses on Medicinal Chemistry, Communicating your research and Proposal writing at Stockholm, Sweden (29th May-1st June, 2012).
Attended FISV (Federazione Italiana Scienze della Vita) 2012 congress, Rome, Italy (24-27th September).
Keystone symposia on Molecular and Cellular Biology, Noncoding RNAs in Development and Cancer, Fairmont Hotel Vancouver, British Columbia, Canada (19th-25th January, 2013) and poster presentation entitled “The role of microRNAs in neural stem cells” by Neha Garg*, Elisabetta Ferretti and Alberto Gulino at the same meeting.
EMBL course on Laboratory animal science VI: Organized by European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo, Rome, Italy (15-26 April, 2013).
diXa: Microarray Analysis using R and Bioconductor course organized by European Molecular Biology Laboratory-European Bioinformatics Institute, CB10 1SD, United Kingdom (14-16 May, 2013).
Healing Summer School and Hedgehog-Gli meeting (Poster Presentation) organized by FP7 Project Healing, Marie Curie, European Union, at Arolla, Switzerland (18-25th August, 2013).
Poster presentation entitled “Epstein-Barr Virus Alters Phenotype of Multiple Myeloma Cells Through Upregulation of miR-21” by Shivangi Yadav, Eleni Anastasiadou, Rachele Bigi, Neha Garg, Elisabetta Ferretti, Luigi Frati, Alberto Faggioni, Pankaj Trivedi at Italian Pathology and Translational Medicine Society (SIPMET) YOUNG SCIENTISTS MEETING, Rome, October23rd-24th, 2013.
Travel Award for 2014 Till & McCulloch Meetings and poster presentation titled “Potential role of STAT3 pathway in regulating tumour initiating cells to and within the central nervous system” by Garg N*, Singh M, Bakhshinyan D, Vora P, Venugopal C, McFarlane N, Manoranjan B, Mahendram S, Gunning P, Singh SK. Organized by Stem Cell Network at Hotel Westin, Ottawa, Canada (October 27-29, 2014), Abstract No. 148.
Poster presentation titled “Pyrvinium selectively targets Glioblastoma brain tumour initiating cells” by C. Venugopal*, R. Hallett, P. Vora, M. Qazi, B. Manoranjan, N. McFarlane, S. Mahendram, M. Singh, N. Garg, J. Hassell and S. Singh. Abstract No. 147. Ottawa, Canada (October 27-29, 2014): Till & McCulloch Meetings. 2014.
Poster presentation titled “Clonal evolution of Medulloblastoma brain tumour-initiating cells (BTICs) in response to therapy: Discovering the refractory BTIC population” by Bakhshinyan D*, Garg N, Venugopal C, Mann A, Vora P, Singh M, van Ommeren R, McFarlane N, Singh Manoranjan B, Qazi M, Scheinemann K, MacDonald P, Delaney K, Whitton A, Dunn S, Singh SK. Abstract No. 134. Ottawa, Canada (October 27-29, 2014): Till & McCulloch Meetings. 2014.
Travel Award for 2015 Till & McCulloch Meetings and poster presentation titled “Potential role of CD133 driven STAT3 pathway in group3 medulloblastoma” by Neha Garg, Robin Hallett, David Bakhshinyan, Thusyanth Vijayakumar, Sujeivan Mahendram, Branavan Manoranjan, Chitra Venugopal, Nicole McFarlane, David Rosa, Patrick Gunning, Sheila K. Singh*. Organized by Stem Cell Network at Sheraton Centre Hotel, Toronto, Canada (October 26-28, 2015), Abstract No. 128.