Dr. Rajanish Giri

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Phone: 01905-267134
EMail: rajanishgiri[at]iitmandi[dot]ac[dot]in
Address: School of Basic Sciences
Indian Institute of Technology Mandi
VPO Kamand, Mandi - 175005
India

Research interests

  • Biophysics, Protein folding and Protein Engineering
  • Intrinsically Disordered Proteins
  • Dark Proteome in viruses and signalling
  • Drug Discovery and Biophysical Study on Zika Virus proteome

Education

  • Ph.D.in Biochemistry from Sapienza University of Rome, Supervisor: Prof. Maurizio Brunori and Carlo Travaglini-Allocatelli, Italy in 2013
  • Master's in Biotechnology from Banaras Hindu University, Varanasi in 2007 ,
  • BSc in Chemistry and Botany from Allahabad University in 2005

Work Experience

Academic Research Experience
  • Assistant Professor, IIT Mandi, Himachal Pradesh, India 2015-till date
  • Postdoctoral Fellow at McMaster University, Hamilton, Canada (2013-2015)
  • Post-doctoral Fellowship from 'Istituto Pasteur-Fondazioni Cenci Bolognetti', Universita Degli Studi di Roma 'La Sapienza' with Prof. Maurizio Brunori (2012-2013)

    • Academic Teaching Experience

    • Course Taught: BY502 - Biophysics and Protein Engineering
    • Course Taught: BY502 - Genetic Engineering
    • Course Taught: BY515- Molecular Biotechnology
    • Course Taught: BY519- Protein Sciences in Therapeutics
    • Course Taught: BY522P- Analytical and Molecular Biotechnology (Practical Course)
    • Course Taught: BY598 & BY599- MTech Thesis Course

      Membership to Professional Bodies

      • F1000 Faculty Specialist
      • Membership: Indian Biophysical Society (life member)
      • Membership: Biophysical Society, USA
      • Membership: American Chemical Society, USA
      • Membership: The Protein Society, USA
      • Membership: American Society for Microbiology (ASM)
      • Membership: Indian Science Congress Association (Life member)
      • Membership: Indian Biophysical Society, India (Life member)
      • Membership: The Biotech Research Society, India (Life Member)
      • Membership: Proteomics Society India (Life Member)
      • Membership: Indian Society of Cell Biology (Life Member)
      • Membership: Society of Biological Chemists (Life Member)
      • Joint Secretary: Bioinformatics and Drug Discovery Society (BIDDS)

Awards, Honors, and Invited Talks

  • Innovative Young Biotechnologist Award, Department of Biotechnology, Govt. of India, (2018)
  • Associate, Indian Academy of Sciences, Bangalore (2017 to 2020)
  • Invited talk in the " 9th National Symposium cum Workshop on "Recent Trends in Structural Bioinformatics and Computer Aided Drug Design"[SBCADD'2017], Algappa University, Karaikudi, Tamilnadu, 14th –17th February, 2017.
  • Invited Talk in the "Bioprocessing India 2016" organized jointly by Center of Innovative and Applied Bioprocessing (CIAB), Mohali, Indian Institute of Science Education and Research (IISER), Mohali and Institute of Microbial Technology (IMTECH), Chandigarh. Mohali, December 15-17, 2016.
  • Invited Talk in the "International Conference on Current Trends in Biotechnology ICCB 2016”, VIT University, Vellore in association with The Biotech Research Society India (BRSI), VIT University, Vellore, December 8-10, 2016.
  • Invited in the "Third International Symposium on Protein Folding and Dynamics”, Organized at National Center for Biological Sciences/ Tata Institute of Fundamental Research, Bangalore, November 8-11, 2016.
  • Invited talk in the " International Conference on Translational Biotechnology (Biosangam 2016)", Organized at MNNIT Allhabad, 4-6 February, 2016.
  • Awarded DST SERB Young Scientist Scheme Grant, 2015.
  • Invited talk in the "Recent Developments in Medical Biotechnology and Structure-Based Drug Designing 2015 (RDMBSBDD-2015), IIT Guwahati Dates: 6-7 December, 2015".
  • Awarded DBT Travel Award to attend International Conference (Gordon Research Conference) on Proteins, Organized at New Hampshire, USA, 14-19 June, 2015.
  • Invited talk in the "5th Annual International Conference on Advances in Biotechnology (Biotech 2015)", Organized at IIT Kanpur, 13-15 March, 2015.
  • Invited talk in the National Symposium on Biophysics & Golden Jubilee Meeting of Indian Biophysical Society, New Delhi, India. (Organized by JMI, Central University, February 14-17, 2015).
  • Awarded with Protein Science Young Investigator Travel Grant / Protein Society Finn Wold Travel Award. July 2013- Poster presentation at The 27th Annual Symposium of The Protein Society, Boston, MA, USA. (July 20–23).
  • Invited talk in the "EMBO workshop on Evolution in the Time of Genomics", Organized by European Molecular Biology Organization, Venice, Italy. 7-9 May, 2012.
  • Awarded fellowship from 'Istituto Pasteur - Fondazioni Cenci Bolognetii, Rome, Italy'
  • Awarded with International Fellowship of Sapienza University of Rome during PhD in Biochemistry.
  • Awarded Junior Research Fellowship from CSIR-UGC (Govt. of India).
  • GATE 2007 in Life Sciences.

Publications

Journal Publications (published)
  • Khrustalev VV*, Giri R, Khrustaleva TA, Kapuganti SK, Stojarov AN, Poboinev VV. Translation-associated mutational U-pressure in the first ORF of SARS-CoV-2 and other coronaviruses bioRxiv. Preprint. 2020.
  • Giri R* Bhardwaj T, Shegane M, Gehi B R, Kumar P, Gadhave K, Oldfield C J, Uversky V N. When Darkness Becomes a Ray of Light in the Dark Times: Understanding the COVID-19 via the Comparative Analysis of the Dark Proteomes of SARS-CoV-2, Human SARS and Bat SARS-Like Coronaviruses bioRxiv Preprint. 2020. (* Corresponding author)
  • A Kumar, P Kumar, S Kumari, VN Uversky, Giri R*. Folding and structural polymorphism of p53 C-terminal domain: One peptide with many conformations. Archives of Biochemistry and Biophysics. 2020 May 15. (* Corresponding author)
  • K Gadhave, Giri R*. Amyloid formation by intrinsically disordered trans-activation domain of cMyb. Biochemical and Biophysical Research Communications. 2020 Jan 30. (* Corresponding author)
  • R Yadav, C Selvaraj, M Aarthy, P Kumar, A Kumar, SK Singh, Giri R*. Investigating into the molecular interactions of flavonoids targeting NS2B-NS3 protease from ZIKA virus through in-silico approaches. Journal of Biomolecular Structure and Dynamics. 2020 Jan 13. (* Corresponding author)
  • Mayank, D Kumar, N Kaur*, Giri R* N Singh*. Biscoumarin scaffold as an efficient anti-Zika virus lead with NS3-Helicase inhibitory potential: in-vitro and in-silico Investigation. New Journal of Chemistry. 2020 Jan 3. (* Corresponding author)
  • Gadhave K, Gehi BR, Kumar P, Xue B, Uversky VN, Giri R*. The dark side of Alzheimer's disease: unstructured biology of proteins from the amyloid cascade signaling pathway. Cell Mol Life Sci. 2020 Jan 2. (* Corresponding author)
  • Khrustalev VV*, Khrustaleva TA, Stojarov AN, Sharma N, Bhaskar B, Giri R Giri R.• The history of mutational pressure changes during the evolution of adeno-associated viruses: A message to gene therapy and DNA-vaccine vectors designers. Infect Genet Evol. 2020 Jan;77:104100.
  • Kumar P, Saumya KU, Giri R*.• Identification of peptidomimetic compounds as potential inhibitors against MurA enzyme of Mycobacterium tuberculosis. J Biomol Struct Dyn. . 2019 Dec 4:1-17 (* Corresponding author)
  • Kumar D, Singh A, Kumar P, Uversky VN, Rao CD*,Giri R*.• Understanding the penetrance of intrinsic protein disorder in rotavirus proteome. Int J Biol Macromol.. 2019 Nov 15. (* Corresponding author)
  • Sharma N, Fonin AV, Shpironok OG, Silonov SA, Turoverov KK, Uversky VN, Kuznetsova IM, Giri R*.• Folding perspectives of an intrinsically disordered transactivation domain and its single mutation breaking the folding propensity. Int J Biol Macromol.. 2019 Nov 13. (* Corresponding author)
  • Kumar D, Aarthy M, Kumar P, Singh SK, Uversky VN, Giri R*.• Targeting the NTPase site of Zika virus NS3 helicase for inhibitor discovery. J Biomol Struct Dyn.. 2019 Nov 14:1-11. (* Corresponding author)
  • Kumar P, Kumar D, Giri R*.• Targeting the nsp2 Cysteine Protease of Chikungunya Virus Using FDA Approved Library and Selected Cysteine Protease Inhibitors. Pathogens. 2019 Aug 15; 8(3). (* Corresponding author)
  • Gour S, Kumar V, Singh A, Gadhave K, Goyal P, Pandey J, Giri R. Yadav JK*.• Mammalian antimicrobial peptide protegrin-4 self assembles and forms amyloid-like aggregates: Assessment of its functional relevance. J Pept Sci. 2019 March;25 (3):e3151.
  • Kumar V, Gour S, Verma N, Kumar S, Gadhave K, Mishra PM, Goyal P, Pandey J, Giri R. Yadav JK*.• The mechanism of phosphatidylcholine-induced interference of PAP (248-286) aggregation. J Pept Sci. 2019 Feb 19:e3152.
  • Kumar D, Sharma N, Aarthy M, Singh S, Giri R*.• Mechanistic insights into Zika virus NS3 helicase inhibition by Epigallocatechin-3-gallate. BiorXiv. 2019. doi: https://doi.org/10.1101/530600. (* Corresponding author)
  • P Kalita, H Shukla, K Gadhave, Giri R. T Tripathi* • Role of the glutaredoxin domain and FAD in the stabilization of thioredoxin glutathione reductase. Archives of biochemistry and biophysics. 2018, Oct 15;656:38-45.
  • Kumar, A, Liang B, Aarthy M, Singh S, Garg N, Mysorekar I, Giri R*. • Hydroxychloroquine inhibits Zika virus NS2B-NS3 protease. ACS Omega. 2018, 3, 18132-18141.(* Corresponding author)
  • Singh A,Kumar A, Uversky VN*, Giri R*. • Understanding the interactability of chikungunya virus proteins via molecular recognition feature analysis. RSC Advances.. 2018,8, 27293-27303.(* Corresponding author)
  • Singh A, Kumar A, Yadav R, Uversky VN, Giri R*. • Deciphering the dark proteome of Chikungunya virus. Scientific Reports. 2018 Apr 11;8(1):5822.(* Corresponding author)
  • Murali A, Kumar D, Giri R ,Singh S K. E7 oncoprotein of human papillomavirus: Structural dynamics and inhibitor screening study. Gene. 2018 (in Press).
  • Kalita J, Shukla R, Shukla H, Gadhave K, Giri R , Tripathi T. Comprehensive analysis of the catalytic and structural properties of a mu-class glutathione s-transferase from Fasciola gigantica. Sci Rep. 2017 Dec 13 doi: 10.1038/s41598-017-17678-3.
  • Mishra PM, Uversky VN*, Giri R*. Molecular Recognition Features in Zika Virus Proteome. Journal of Molecular Biology. 2017 November 7 doi: 10.1016/j.jmb.2017.10.018.(* Corresponding Authors)
  • Sharma N, Murali A, Singh SK*, Giri R*. Epigallocatechin gallate, an active green tea compound inhibits the Zika virus entry into host cells via binding the envelope protein. Int J Biol Macromol.. 2017 June 28; 104(Pt A): 1046-1054(* Corresponding Authors)
  • Kumar D, Sharma N and Giri R*. Therapeutic Interventions of Cancers Using Intrinsically Disordered Proteins as Drug Targets: c-Myc as Model System. Cancer Inform. 2017 March 16(* Corresponding Author)
  • Khrustalev VV*, Khrustaleva TA,Sharma N and Giri R*. Mutational Pressure in Zika Virus:Local ADAR-Editing Areas Associated with Pauses in Translation and Replication. Front Cell Infect Microbiol.. 2017 Feb 22;7:44.(* Corresponding Authors)
  • Giri R*, Kumar D, Sharma N, Uversky VN*. Intrinsically Disordered Side of the Zika Virus Proteome. Front Cell Infect Microbiol.. 2016 Nov 4;6:144.(* Corresponding Authors)
  • Camilloni C, Bonetti D, Morrone A, Giri R, Dobson CM, Brunori M, Gianni S, Vendruscolo M. Towards a structural biology of the hydrophobic effect in protein folding. Sci Rep. 2016 Jul 27;6:28285.
  • Toto A, Camilloni C, Giri R, Brunori M, Vendruscolo M, Gianni S. Molecular Recognition by Templated Folding of an Intrinsically Disordered Protein. Sci Rep. 2016 Feb 25;6:21994.
  • Dey G, Gaur P, Giri R*, Ghosh S*. Optical signaling in biofluids: a nondenaturing photostable molecular probe for serum albumins. Chem Commun. 2016 Jan 21;52(9):1887-90. (* Corresponding Authors)
  • VanSchouwen B, Selvaratnam R, Giri R, Lorenz R, Herberg FW, Kim C, Melacini G. " Mechanism of cAMP Partial Agonism in Protein Kinase G (PKG).J Biol Chem, 2015 Nov 27;290(48):28631-41.
  • Gianni S, Camilloni C, Giri R , Toto A, Bonetti D, Morrone A, Sormanni P, Brunori M, Vendruscolo M. “Understanding the frustration arising from the competition between function, misfolding and aggregation in a globular protein”. Proc Natl Acad Sci U S A. 2014 Sep 30;111(39):14141-6.
  • Toto A, Giri R, Brunori M, and Gianni S. " The mechanism of binding of the KIX domain to the Mixed Lineage Leukemia protein and its allosteric role in the recognition of c-Myb ". Protein Sci. 2014;23(7):962-9.
  • Boulton S, Akimoto M, Vanschouwen B, Moleschi K, Selvaratnam R, Giri R, Melacini G. " Tapping the translation potential of cAMP signalling: molecular basis for selectivity in cAMP agonism and antagonism as revealed by NMR ". Biochemical Society transactions, 2014, 42(2):302-7.
  • Bonetti D, Toto A, Giri R, Morrone A, Sanfelice D, Pastore A, Temussi P, Gianni S and Brunori M. "The kinetics of folding of frataxin". Physical Chemistry Chemical Physics 2014 Apr 14, 16:6391-7.
  • Giri R, Travaglini-Allocatelli C, Gianni S, Giardina G and Brunori M. ""Morphogenesis of proteins: classic views and challenges ahead". Journal of Proteins and Proteomics, 2013.
  • Giri R, Morrone A, Toto A, Brunori M, Gianni S, “Structure of the transition state for the binding of c-Myb and KIX highlights an unexpected order for a disordered system”. Proc Natl Acad Sci U S A. 2013,110(37):14942-7.
  • Gianni S , Morrone A , Giri R , Brunori M, "A folding-after-binding mechanism describes the recognition between the transactivation domain of c-Myb and the KIX domain of the CREB-binding protein.", Biochemical and biophysical research communications, 2012, 428(2).
  • Morrone A , Giri R , Brunori M , Gianni S, "Reassessing the folding of the KIX domain: evidence for a two-state mechanism.", Protein science , 2012, 21(11).
  • Giri R , Morrone A , Travaglini-Allocatelli C , Jemth P , Brunori M , Gianni S, "Folding pathways of proteins with increasing degree of sequence identities but different structure and function.", Proc Natl Acad Sci U S A. 2012;109(44):17772-6.
  • Brunori M , Gianni S , Giri R , Morrone A , Travaglini-Allocatelli C, "Morphogenesis of a protein: folding pathways and the energy landscape.", Biochemical Society Transactions, 2012, 40(2).
  • Morrone A , Giri R , Toofanny RD , Travaglini-Allocatelli C , Brunori M , Daggett V , Gianni S, "GB1 is not a two-state folder: identification and characterization of an on-pathway intermediate.", Biophysical Journal, 2011, 101(8).

    • Conferences and Symposia

    • February 2015: Invited lecture in the National Symposium on Biophysics & Golden Jubilee Meeting of Indian Biophysical Society, New Delhi, India. (February 14-17).
    • July 2013- Poster presentation at The 27th Annual Symposium of The Protein Society, Boston, MA, USA. (July 20–23). Awarded with Protein Science Young Investigator Travel Grant / Protein Society Finn Wold Travel Award.
    • May 2012- Oral and poster presentation at the EMBO workshop on “Evolution in the time of genomics”, EMBO, Venice, Italy. (May 7-9)
    • June 2011- Poster presentation in the FEBS symposium on “Biochemistry for tomorrow’s medicine”, FEBS, Turin, Italy. (June 25-30)
    • December 2010- Participation in the International symposium on “Protein Structure and Dynamics”, Accademia Nazionale dei Lincei, Rome, Italy. (December 1-3)
    • November 2008- I participated in National seminar and workshop on “METABOLIC NETWORKS AND DRUG DESIGNING” organized by Dept. of Biotechnology, Government of India, at Varanasi, India. (November 28-30)
    • November 2008- I participated in “4th ASIA OCEANIA CONFERENCE ON PHOTOBIOLOGY” organized by Banaras Hindu University, Varanasi, India. (November 24-26)
    • March 2008- Poster presentation at Indo-Danish symposium, held at University of Delhi (India). (March 17-18)

    Job Opportunities

    • We encourage the applications for postdoctoral positions through National Postdoctoral Programs. Post Doctoral Fellows/Research Associates with their Own Grants/fellowships are welcome to contact via E-mail with your full bio-data, previous works, best three publications and one page write up on the proposed plan.
    .