Metabolic Systems Biology - Metabolomics and 13C fluxomics
Metabolism of Plant and microbial systems
Cellular Bioprocessing Technology towards sustainable generation of high value chemicals
Modern tools and techniques for probing metabolism - NMR, GC-MS, metabolic modelling etc
Education
Ph.D. in Plant Sciences (13C Fluxomics,GC-MS, NMR), University of Oxford, UK (2007-2011, Awarded 2012).
Supervisors: Prof. George Ratcliffe and Prof. Nick Kruger
M.Tech in Agri. Biotech. (Applied Botany), Indian Institute of Technology, Kharagpur (2005-2007)
PG Diploma in Bioinformatics (full time), Amity Inst. of Biotechnology, Noida (2001-2003)
M.Sc in Botany (Biochemistry and Advanced Plant physiology as majors), Berhampur University, Odisha (1999-2001)
B.Sc in Botany (Honours),Rayagada College, Berhampur University, Orissa, (1996-1999)
Work Experience
Academic Research Experience
Associate Professor, IIT Mandi, Himachal Pradesh, India (Sept 2019 - Present)
Assistant Professor, IIT Mandi, Himachal Pradesh, India (Feb 2015 - Sept 2019)
Postdoctoral Research Associate, University of Bath, UK (Sept 2012 - Feb 2015)
Postdoctoral Assistant, University of Oxford, UK (Feb 2011 - July 2012)
Teaching Experience
Lecturer, Department of Botany (UGC vacancy, Govt. of India), Rayagada College, Berhampur University, Odisha (Aug 2004 - July 2005).
Lecturer in the Division of Biotechnology, MITS Engineering College, Odisha (Feb 2003 - Aug 2004)
Awards and Honors
Awarded Grants of Nationally relevant high impact projects - DBT-BMBF, IMPRINT, SPARC, FarmerZone
Distinguished Teacher Award (2017)-IIT Mandi for sustained performance of excellence in teaching
Early Career Research Award: SERB–ECR 2017-2020
Awarded Clarendon Scholarship for pursuing D.Phil at University of Oxford (2007-10)
Received Overseas Research Student (ORS) Award, University of Oxford (2007-10)
Received Mr Krishna Pathak Scholarship, Exeter College, University of Oxford (2007-10)
Received travel award from Society for Experimental Biology for presenting research work in international conference, SEB Glasgow 2012
Received travel award from Exeter College, University of Oxford for presenting my work in Plant Systems Biology UK workshop, Nottingham, June 1-3, 2010
Awarded fellowship from MHRD, India for pursuing M.Tech at IIT Kharagpur (2005-07)
Sponsored candidate by MITS engineering college for pursuing PGD in Bioinformatics
Qualified GATE-2005 (Graduate aptitude test in engineering) conducted by IITs and IISc
Class topper in M.Tech and P.G.D. in Bioinformatics – Awarded prizes
Qualified CSIR-UGC National Eligibility Test (NET), 2001 for lectureship
Review editor for the journals ‘Frontiers – Plant Systems Biology’ and “Plant Chemodiversity”
Reviewed for journals Physiologia plantarum, Phytochemistry, Waste management, International Journal of Biological Macromolecules
Reviewed project proposals from DBT, BBSRC (UK) and DFG (Germany)
Publications
Journal Publications (published)
Pandey S, Kumari A, Shree M, Kumar V, Singh P, Bharadwaj C, Loake G, Parida SK, Masakapalli SK, and Jagadis K Gupta (2019). Nitric oxide accelerates germination via the regulation of respiration in chickpea. Journal of Experimental Botany, erz185.
Yadav A, Bakshi S, Yadukrishnan P, Lingwan M, Dolde U, Wenkel S, Masakapalli SK, Datta S (2019). The B-box-containing microprotein miP1a/BBX31 regulates photomorphogenesis and UV-B protection. Plant physiology, pp. pp-01258.
Yadav, A, Lingwan, M, Yadukrishnan, PS, Masakapalli, SK*., Datta S* (2019). BBX31 promotes hypocotyl growth, primary root elongation and UV-B tolerance in Arabidopsis. Plant Signaling & Behavior, 5:1-3. (*corresponding author)
Shree M, Lingwan, M., Masakapalli SK* (2019). Metabolite Profiling and Metabolomics of Plant Systems Using 1H NMR and GC-MS. OMICS-Based Approaches in Plant Biotechnology, 129-144, John Wiley & Sons, Inc. (*corresponding author)
Shree M, Masakapalli SK* (2018). Intracellular Fate of Universally Labelled 13C Isotopic Tracers of Glucose and Xylose in Central Metabolic Pathways of Xanthomonas oryzae. Metabolites, 8 (4), 66 (*corresponding author)
Pfau T, Christian N, Masakapalli SK, Sweetlove LJ, Poolman MG, Ebenhöh O (2018). The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling. Scientific reports 8 (1), 12504
Semwal T, Masakapalli SK, Kala VU (2018). Root Morphology and Mechanical Characteristics of Himalayan (Indian) Native Plant Species. The International Congress on Environmental Geotechnics, 385-392 [Conference Paper]
Kruger NJ, Masakapalli SK and Ratcliffe RG (2017). Assessing metabolic flux in plants with radiorespirometry. In Plant Respiration and Internal Oxygen: Methods and Protocols (ed K.J. Gupta) Methods in Molecular Biology (Clifton, N.J., Springer) 1670:1-16 [Book chapter]
Terpolilli J, Masakapalli SK et al.,...Poole P (2016). Lipogenesis and redox balance in nitrogen-fixing pea bacteroids.Journal of Bacteriology 198 (20): 2864-2875
Masakapalli SK, Kruger NJ, Ratcliffe RG (2014). The metabolic flux phenotype of heterotrophic Arabidopsis cells reveal a flexible balance between the cytosolic and plastidic contributions to carbohydrate oxidation in response to phosphate limitation.The Plant Journal, 78(6):964-77.
Masakapalli SK, Ratcliffe RG, Anneli R, Kirsi-Marja O, Sweetlove LJ (2014). Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production.Phytochemistry, 99:73-85.
Masakapalli SK, Ratcliffe RG, Williams TCR (2014). Quantification of 13C enrichments and isotopomer abundances for metabolic flux analysis using NMR spectroscopy.Methods in Molecular Biology, 1090:73-86.
Kruger NJ, Masakapalli SK* and Ratcliffe RG (2014). Optimisation of steady-state 13C-labelling experiments for metabolic flux analysis.Methods in Molecular Biology, 1090:53-72 (*Corresponding author).
Masakapalli SK, Kruger NJ, Ratcliffe RG (2013). The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a complex response to changes in nitrogen supply.The Plant Journal, 74(4): 569–582.
Gupta SD, Masakapalli SK (2013). Mushroom tyrosinase inhibition activity of Aloe vera gel from different germplasms.Chinese journal of natural medicines, 11 (6): 616-620.
Kruger NJ, Masakapalli SK and Ratcliffe RG (2012). Strategies for investigating the plant metabolic network with steady-state metabolic flux analysis: lessons from an Arabidopsis cell culture and other systems.Journal of Experimental Botany, 63(6):2309-23. (Special issue in Pathways & Fluxes)
Masakapalli SK, Le Lay P, Huddleston JE, Pollock NL, Kruger NJ, Ratcliffe RG (2010). Subcellular flux analysis of central metabolism in a heterotrophic Arabidopsis cell suspension using steady-state stable isotope labelling.Plant Physiology, 152: 602-619 (Plant Systems Biology Focus issue).
Masakapalli SK, Datta PK, Gupta SD (2009). In vitro evaluation of UV opacity potential of Aloe vera L. gel from different germplasms.Journal of Natural Medicines, 63: 195–199.
Williams TCR, Miguet L, Masakapalli SK, Kruger NJ, Sweetlove LJ, Ratcliffe RG (2008). Metabolic network fluxes in heterotrophic Arabidopsis cells: Stability of the flux distribution under different oxygenation conditions.Plant Physiology, 148:704-718.
Conferences and Symposia
Oral presentations
Masakapalli SK. Defining cellular metabolic phenotypes using 13C tracers, NMR and GC-MS, Presented at Institute of Microstructure, Karlsruhe Institute of Technology, Germany, 2nd Aug 2018
Masakapalli SK. 13C fluxomics – challenges and opportunities to define cellular metabolic phenotypes. Presented at BESCON-2018, IIT Bombay, 27th Oct 2018
Masakapalli SK. Introducing 13C Fluxomics and Smart Agriculture in Indian Context. Presented at NCBS, Bangalore, 20th Nov 2018
Masakapalli SK. Comprehensive Phytochemical analysis from Himalayan flora with their potential applications at The Himalaya Drug Company, Bangalore, 19th Nov 2018
Masakapalli SK. Introducing Metabolomics, Fluxomics and Smart Agriculture in Indian context. Presented at IISER Bhopal, 27th Dec 2018
Masakapalli SK. High Tech Agriculture for Indian farms - How can we contribute? Presented at Rayagada College, Odisha, 16th Jan 2019
Masakapalli SK. 13C fluxomics towards mapping the Plant-microbial metabolic cross talk. Presented at Molecular Intricacies of plant associated microorganisms, MIPAM-2019, NIPGR, New Delhi, Feb 1st to 3rd, 2019
Masakapalli SK. Metabolic Systems Biology approaches for optimal bioprocessing of cellulosic waste to valuables. Presented at BioSD, IICT Hyderabad, 24th Nov 2018
Masakapalli SK, Ratcliffe RG, Sweetlove LJ. Metabolic flux phenotypes of transgenic tobacco hairy roots engineered for increased production of terpenoid pharmaceuticals (flash talk) enGENEious - Evolving Life for Future Technologies, University of Oxford, UK (http://engeneious.chem.ox.ac.uk); 06/2012
Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different nitrogen sources. 2011 CRS Symposium, Dept of Plant Sciences, Oxford, Sept 27, 2011 (oral presentation)
Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different levels of inorganic phosphate. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th), (oral presentation)
Masakapalli SK, Kruger NJ, Ratcliffe RG. Network flux analysis in plants: experimental and modeling strategies to analyse compartmented and perturbed systems. Plant Systems Biology UK workshop, Nottingham, June 1-3, 2010 (20 min oral presentation)
Posters
Masakapalli SK, Ratcliffe RG, Sweetlove LJ. Metabolic flux phenotypes of transgenic tobacco hairy roots engineered for increased production of terpenoid pharmaceuticals (poster) enGENEious - Evolving Life for Future Technologies, University of Oxford, UK (http://engeneious.chem.ox.ac.uk); 06/2012
Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different nitrogen sources. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th)
Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different levels of inorganic phosphate. SEB Glasgow 2011-Pathways and fluxes session (July 2- 4th)
Masakapalli SK, Terpolilli JJ, Poole PS, Kruger NJ, Ratcliffe RG. Metabolic flux analysis of free living Rhizobium leguminosarum. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th)
McCraw SL, Masakapalli SK, Kruger NJ, Ratcliffe RG, Preston GM. Metabolic analysis of Pseudomonas syringae 8th International Conference on Pseudomonas syringae and related Pathogens, 31 Aug - 03 Sept 2010 (poster presented by SLM)
Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells under phosphorus stress. International conference on Systems Biochemistry, University of York, March 21-23, 2010 (poster presented by NJK and RGR)
Masakapalli SK, Kruger NJ, Ratcliffe RG. Impact of phosphate starvation on central metabolism in Arabidopsis cell culture. Graduate student symposium ‘Food security in the 21st century’ Department of Plant Sciences, University of Oxford, 5th-9th Oct 2009
Masakapalli SK, Datta PK, Gupta SD, Anti-tyrosinase and UV-opacity potential of Aloe vera L. gel from different germplasms. National seminar on plant physiology, 28-30 Nov 2006, Kerala Agricultural University, India.
Workshop/Conferences Organised
Two days FarmerZone 2018 workshop (May 2018) co-organised along with Farmerzone team at IIT Mandi – Participants were Principal Scientific Advisor, Govt of India; DBT India; CPRI Shimla and Meerut, NIAB UK; IORA Ecological solutions, Delhi; Vertiver, Delhi; Lahoul Potato Growers Association, Manali; Sanga Farms, Jalandhar; TCS; Microsoft Research, India and GODAN
External links of professional profile
Research Gate: http://www.researchgate.net/profile/Shyam_Masakapalli
Google Scholar: http://scholar.google.co.in/citations?user=UnAJBy4AAAAJ&hl=en