Dr. Shyam Kumar Masakapalli

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Phone: +91-1905-237975
EMail: shyam@iitmandi.ac.in
Address: School of Basic Sciences
Indian Institute of Technology Mandi,
Near Bus Stand, Mandi - 175001
Himachal Pradesh, India

Research interests

  • Metabolic network biology - fluxomics, metabolomics and transcriptomics (RNAseq) of cellular systems
  • Central metabolism of Plant and microbial systems
  • Biochemistry, Physiology and Metabolic engineering of microbes for sustainable generation of high value chemicals
  • Modern tools and techniques for probing metabolism - NMR, GC-MS, metabolic modelling etc
  • Natural product biotechnology – secondary metabolic pathways in plants
  • Development of Microbial and plant hairy root systems as cell factories
  • Plant microbial interaction at the level of metabolism

Education

  • Ph.D. in Plant Sciences (13C Fluxomics,GC-MS, NMR), University of Oxford, UK (2007-2011, Awarded 2012). Supervisors: Prof. George Ratcliffe and Dr. Nick Kruger
  • M.Tech in Agri. Biotech. (Applied Botany), Indian Institute of Technology, Kharagpur (2005-2007)
  • PG Diploma in Bioinformatics (full time), Amity Inst. of Biotechnology, Noida (2001-2003)
  • M.Sc in Botany (Biochemistry and Advanced Plant physiology as majors), Berhampur University, Odisha (1999-2001)
  • B.Sc in Botany (Honours),Rayagada College, Berhampur University, Orissa, (1996-1999)

Work Experience

Academic Research Experience
  • Assistant Professor, IIT Mandi, Himachal Pradesh, India (Feb 2015 - Present)
  • Postdoctoral Research Associate, University of Bath, UK (Sept 2012 - Feb 2015)
  • Postdoctoral Assistant, University of Oxford, UK (Feb 2011 - July 2012)
Teaching Experience
  • Lecturer, Department of Botany (UGC vacancy, Govt. of India), Rayagada College, Berhampur University, Odisha (Aug 2004 - July 2005).
  • Lecturer in the Division of Biotechnology, MITS Engineering College, Odisha (Feb 2003 - Aug 2004)

Awards and Honors

  • Awarded Clarendon Scholarship for pursuing D.Phil at University of Oxford (2007-10)
  • Received Overseas Research Student (ORS) Award, University of Oxford (2007-10)
  • Received Mr Krishna Pathak Scholarship, Exeter College, University of Oxford (2007-10)
  • Received travel award from Society for Experimental Biology for presenting research work in international conference, SEB Glasgow 2012
  • Received travel award from Exeter College, University of Oxford for presenting my work in Plant Systems Biology UK workshop, Nottingham, June 1-3, 2010
  • Awarded fellowship from MHRD, India for pursuing M.Tech at IIT Kharagpur (2005-07)
  • Sponsored candidate by MITS engineering college for pursuing PGD in Bioinformatics
  • Qualified GATE-2005 (Graduate aptitude test in engineering) conducted by IITs and IISc
  • Class topper in M.Tech and P.G.D. in Bioinformatics – Awarded prizes
  • Qualified CSIR-UGC National Eligibility Test (NET), 2001 for lectureship
  • Review editor for the journals ‘Frontiers – Plant Systems Biology’ and ‘PLOS-One’

Publications

Journal Publications (published)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG (2014) The metabolic flux phenotype of heterotrophic Arabidopsis cells reveal a flexible balance between the cytosolic and plastidic contributions to carbohydrate oxidation in response to phosphate limitation. The Plant Journal, 78(6):964-77.
  • Masakapalli SK, Ratcliffe RG, Anneli R, Kirsi-Marja O, Sweetlove LJ (2014) Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production. Phytochemistry, 99:73-85.
  • Masakapalli SK, Ratcliffe RG, Williams TCR (2014) Quantification of 13C enrichments and isotopomer abundances for metabolic flux analysis using NMR spectroscopy. Methods in Molecular Biology, 1090:73-86.
  • Kruger NJ, Masakapalli SK* and Ratcliffe RG (2014) Optimisation of steady-state 13C-labelling experiments for metabolic flux analysis. Methods in Molecular Biology, 1090:53-72 (*Corresponding author).
  • Masakapalli SK, Kruger NJ, Ratcliffe RG (2013) The metabolic flux phenotype of heterotrophic Arabidopsis cells reveals a complex response to changes in nitrogen supply. The Plant Journal, 74(4): 569–582.
  • Gupta SD, Masakapalli SK (2013) Mushroom tyrosinase inhibition activity of Aloe vera gel from different germplasms. Chinese journal of natural medicines, 11 (6): 616-620.
  • Kruger NJ, Masakapalli SK and Ratcliffe RG (2012) Strategies for investigating the plant metabolic network with steady-state metabolic flux analysis: lessons from an Arabidopsis cell culture and other systems. Journal of Experimental Botany, 63(6):2309-23. (Special issue in Pathways & Fluxes)
  • Masakapalli SK, Le Lay P, Huddleston JE, Pollock NL, Kruger NJ, Ratcliffe RG (2010) Subcellular flux analysis of central metabolism in a heterotrophic Arabidopsis cell suspension using steady-state stable isotope labelling. Plant Physiology, 152: 602-619 (Plant Systems Biology Focus issue).
  • Masakapalli SK, Datta PK, Gupta SD (2009) In vitro evaluation of UV opacity potential of Aloe vera L. gel from different germplasms. Journal of Natural Medicines, 63: 195–199.
  • Williams TCR, Miguet L, Masakapalli SK, Kruger NJ, Sweetlove LJ, Ratcliffe RG (2008). Metabolic network fluxes in heterotrophic Arabidopsis cells: Stability of the flux distribution under different oxygenation conditions. Plant Physiology, 148:704-718.

Conferences and Symposia

Oral presentations
  • Masakapalli SK, Ratcliffe RG, Sweetlove LJ. Metabolic flux phenotypes of transgenic tobacco hairy roots engineered for increased production of terpenoid pharmaceuticals (flash talk) enGENEious - Evolving Life for Future Technologies, University of Oxford, UK (http://engeneious.chem.ox.ac.uk); 06/2012
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different nitrogen sources. 2011 CRS Symposium, Dept of Plant Sciences, Oxford, Sept 27, 2011 (oral presentation)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different levels of inorganic phosphate. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th), (oral presentation)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Network flux analysis in plants: experimental and modeling strategies to analyse compartmented and perturbed systems. Plant Systems Biology UK workshop, Nottingham, June 1-3, 2010 (20 min oral presentation)
Posters
  • Masakapalli SK, Ratcliffe RG, Sweetlove LJ. Metabolic flux phenotypes of transgenic tobacco hairy roots engineered for increased production of terpenoid pharmaceuticals (poster) enGENEious - Evolving Life for Future Technologies, University of Oxford, UK (http://engeneious.chem.ox.ac.uk); 06/2012
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different nitrogen sources. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells grown on different levels of inorganic phosphate. SEB Glasgow 2011-Pathways and fluxes session (July 2- 4th)
  • Masakapalli SK, Terpolilli JJ, Poole PS, Kruger NJ, Ratcliffe RG. Metabolic flux analysis of free living Rhizobium leguminosarum. SEB Glasgow 2011-Pathways and fluxes session (July 2nd - 4th)
  • McCraw SL, Masakapalli SK, Kruger NJ, Ratcliffe RG, Preston GM. Metabolic analysis of Pseudomonas syringae 8th International Conference on Pseudomonas syringae and related Pathogens, 31 Aug - 03 Sept 2010 (poster presented by SLM)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Metabolic flux phenotypes of Arabidopsis cells under phosphorus stress. International conference on Systems Biochemistry, University of York, March 21-23, 2010 (poster presented by NJK and RGR)
  • Masakapalli SK, Kruger NJ, Ratcliffe RG. Impact of phosphate starvation on central metabolism in Arabidopsis cell culture. Graduate student symposium ‘Food security in the 21st century’ Department of Plant Sciences, University of Oxford, 5th-9th Oct 2009
  • Masakapalli SK, Datta PK, Gupta SD, Anti-tyrosinase and UV-opacity potential of Aloe vera L. gel from different germplasms. National seminar on plant physiology, 28-30 Nov 2006, Kerala Agricultural University, India.

External links of professional profile

  • Research Gate: http://www.researchgate.net/profile/Shyam_Masakapalli
  • Google Scholar: http://scholar.google.co.in/citations?user=UnAJBy4AAAAJ&hl=en
  • LinkedIn: https://uk.linkedin.com/pub/shyam-kumar-masakapalli/8/311/2
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