#!/usr/bin/perl -w use strict; use warnings; use CGI qw(:standard); print "Content-Type: text/html\n\n"; print< G2KO tool

G2KO pipeline tool by MetSysBioLab,IIT Mandi

 G2KO pipeline tool can process KO identifiers based on KEGG annotation.


Step 1. If you have 3/4 letter organism KEGG codes ready, skip to step 3.

Step 2. If you dont have organism codes, download the currently available list of organism names and codes from G2KO home page or click here. You will be prompted to save a text file. Save it on your desired location on your computer. Search the file by opening in any text editor like notpad or gedit or textpad or wordpad and search the file (control+F on windows and linux, cmd+F on MacOs)

Step 3. First, input the number of organisms you want to compare. Note: The tool does not restrict the number of organisms, however the KEGG Mapper can only accept KO identifiers of 10 organisms. After entering the desired number of organisms, click 'Next' or press 'Enter'

Step 4. Write the organism names and organism's KEGG codes in the respective text fields. If you are not sure about organism's KEGG codes, refer to step 2 to obtain the text file containing the listof organisms. After filling all the fields, press 'Download KO identifiers' button or press 'Enter'.

Step 5. You will be prompted to save a text file, by default named output.txt. Save this file to your computer. This step takes from few seconds to few minutes depending on your number or organisms and internet connection. Please be patient, average wait time for 10 organisms is around a minute. This 'output.txt' file contains all the KO numbers of your selected organisms in proper format.

Step 6. Upload the text file you downloaded in step 3, without modifications, to KEGG mapper pathway reconstruction here. The link to get to KEGG mapper pathway reconstruct tool is also provided on the results page.





Contact:
Chandrakant Joshi
PhD scholar, under supervision of Dr. Shyam Kumar Masakapalli
Metabolic Systems Biology Lab,
BioX centre, School of Basic Sciences,
IIT Mandi, Himachal Pradesh 175005
chandrakant.iitmandi@gmail.com
7415292666



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